El papel dual de los biofilms en la exploración espacial
DOI:
https://doi.org/10.69976/aspast.v2n2.3Keywords:
Comunidades microbianas, hábitats espaciales, microgravedad, reciclaje biológico, sistemas autosuficientesAbstract
Las comunidades microbianas adheridas a superficies representan un componente inevitable de los hábitats espaciales y muestran respuestas fisiológicas particulares bajo condiciones de microgravedad, incluyendo un aumento en la complejidad estructural y en la tolerancia al estrés. Estas características incrementan los riesgos asociados a la degradación de materiales, la alteración de sistemas críticos y la salud de las tripulaciones en entornos cerrados. Sin embargo, dichas propiedades también abren oportunidades para su aprovechamiento en procesos de reciclaje, producción de materiales y mantenimiento de sistemas autosuficientes durante misiones de larga duración. Este trabajo analiza de forma sintética esta dualidad funcional, resaltando la importancia de estrategias de control y uso dirigido en el contexto de la exploración humana más allá de la Tierra.
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Ali, A., Zahra, A., Kamthan, M., Husain, F. M., Albalawi, T., Zubair, M., Alatawy, R., Abid, M., & Noorani, M. S. (2023). Microbial Biofilms: Applications, Clinical Consequences, and Alternative Therapies. Microorganisms, 11(8), 1934. https://doi.org/10.3390/microorganisms11081934
Berliner, A. J., Hilzinger, J. M., Abel, A. J., McNulty, M., Makrygiorgos, G., & Cumbers, J. (2021). Towards a biomanufactory on Mars. Frontiers in Astronomy and Space Sciences, 8, 120. https://doi.org/10.3389/fspas.2021.711550
Branda, S. S., Vik, S., Friedman, L., & Kolter, R. (2005). Biofilms: the matrix revisited. Trends in microbiology, 13(1), 20–26. https://doi.org/10.1016/j.tim.2004.11.006
Characklis, W. G. (1973). Attached microbial growths—II. Frictional resistance due to microbial slimes. Water Research, 7, 1249–1258. https://doi.org/10.1016/0043-1354(73)90002-X
Checinska Sielaff, A., Urbaniak, C., Mohan, G. B. M., Stepanov, V. G., Tran, Q., Wood, J. M., ... & Venkateswaran, K. (2019). Characterization of the total and viable bacterial and fungal communities associated with the International Space Station surfaces. Microbiome, 7(1), 50. https://doi.org/10.1186/s40168-019-0666-x
Cockell, C. S. (2022). Bridging the gap between microbial limits and extremes in space: space microbial biotechnology in the next 15 years. Microbial Biotechnology, 15(1), 29-41. https://doi.org/10.1111/1751-7915.13927
Cortesão, M., et al. (2020). Fungal biotechnology in space: Why and how? Applied Microbiology and Biotechnology, 104, 7409–7421. https://doi.org/10.1007/978-3-030-29541-7_18
Costerton, J. W., Geesey, G. G., & Cheng, K. J. (1978). How bacteria stick. Scientific American, 238(1), 86–95. https://doi.org/10.1038/scientificamerican0178-86
Crabbé, A., Pycke, B., Van Houdt, R., Monsieurs, P., Nickerson, C., Leys, N., & Cornelis, P. (2010). Response of Pseudomonas aeruginosa PAO1 to low shear modelled microgravity involves AlgU regulation. Environmental microbiology, 12(6), 1545–1564. https://doi.org/10.1111/j.1462-2920.2010.02184.x
Crucian, B., Babiak-Vazquez, A., Johnston, S., Pierson, D. L., Ott, C. M., & Sams, C. (2018). Immune system dysregulation during spaceflight. Frontiers in Immunology, 9, 1437. https://doi.org/10.3389/fimmu.2018.01437
De Micco, V., Amitrano, C., Mastroleo, F., Aronne, G., Battistelli, A., Carnero-Diaz, E., ... & Leys, N. (2023). Plant and microbial science and technology as cornerstones to Bioregenerative Life Support Systems in space. npj Microgravity, 9(1), 69.
Dhami, N. K., Reddy, M. S., & Mukherjee, A. (2013). Biomineralization of calcium carbonates and their engineered applications: a review. Frontiers in Microbiology, 4, 314. https://doi.org/10.3389/fmicb.2013.00314
Dikshit, R., Gupta, N., Dey, A., Viswanathan, K., & Kumar, A. (2022). Microbial induced calcite precipitation can consolidate martian and lunar regolith simulants. Plos one, 17(4), e0266415. https://doi.org/10.1371/journal.pone.0266415
Donato, V. (2018). El mundo de los biofilms. Editorial Autores de Argentina.
Donlan, R. M. (2002). Biofilms: Survival mechanisms of clinically relevant microorganisms. Clinical Microbiology Reviews, 15(2), 167–193. https://doi.org/10.1128/CMR.15.2.167-193.2002
Flemming, H. C., Wingender, J., Szewzyk, U., Steinberg, P., Rice, S. A., & Kjelleberg, S. (2016). Biofilms: An emergent form of bacterial life. Nature Reviews Microbiology, 14(9), 563–575. https://doi.org/10.1038/nrmicro.2016.94
Godia, F., Albiol, J., Montesinos, J. L., Pérez, J., Creus, N., Cabello, F., Mengual, X., & Montras, A. (2002). MELISSA: A loop of interconnected bioreactors to develop life support in space. Journal of Biotechnology, 99(3), 319–330. https://doi.org/10.1016/S0168-1656(02)00222-5
Gouveia, A. G., Taoufiq, A., Antunes, W., & Romão, C. V. (2025). Metal–induced biofilm formation by radiation resistant bacteria Deinococcus radiodurans and Deinococcus indicus. Extremophiles, 29(3), 38.
Guerrero, A. C. (2023). Images and the development of the microbial biofilm concept (Doctoral dissertation). Arizona State University.
Guo, Q., Zhan, Y., Zhang, W., Wang, J., Yan, Y., Wang, W., & Lin, M. (2023). Development and regulation of the extreme biofilm formation of Deinococcus radiodurans R1 under Extreme environmental conditions. International Journal of Molecular Sciences, 25(1), 421.
Hall-Stoodley, L., & Stoodley, P. (2009). Evolving concepts in biofilm infections. Cellular Microbiology, 11(7), 1034–1043. https://doi.org/10.1111/j.1462-5822.2009.01323.x
Hendrickx, L., De Wever, H., Hermans, V., Mastroleo, F., Morin, N., Wilmotte, A., Janssen, P., & Mergeay, M. (2006). Microbial ecology of the closed artificial ecosystem MELiSSA (Micro-Ecological Life Support System Alternative): reinventing and compartmentalizing the Earth's food and oxygen regeneration system for long-haul space exploration missions. Research in microbiology, 157(1), 77–86. https://doi.org/10.1016/j.resmic.2005.06.014
Heukelekian, H., & Heller, A. (1940). Relation between Food Concentration and Surface for Bacterial Growth. Journal of bacteriology, 40(4), 547–558. https://doi.org/10.1128/jb.40.4.547-558.1940
Horneck, G., Klaus, D. M., & Mancinelli, R. L. (2010). Space microbiology. Microbiology and molecular biology reviews, 74(1), 121-156.
Huang, B., Li, D. G., Huang, Y., & Liu, C. T. (2018). Effects of spaceflight and simulated microgravity on microbial growth and secondary metabolism. Military Medical Research, 5(1), 18. https://doi.org/10.1186/s40779-018-0162-9
Hupka, M., Kedia, R., Schauer, R., Shepard, B., Granados-Presa, M., Vande Hei, M., Flores, P., & Zea, L. (2023). Morphology of Penicillium rubens Biofilms Formed in Space. Life, 13(4), 1001. https://doi.org/10.3390/life13041001
Justiniano, Y. A. V., Goeres, D. M., Sandvik, E. L., Kjellerup, B. V., Sysoeva, T. A., Harris, J. S., & HerrNeckar, L. E. (2023). Mitigation and use of biofilms in space for the benefit of human space exploration. Biofilm, 5, 100102. https://doi.org/10.1016/j.bioflm.2022.100102
Kim, W., Tengra, F. K., Young, Z., Shong, J., Marchand, N., Chan, H. K., Pangule, R. C., Parra, M., Dordick, J. S., & Plawsky, J. L. (2013). Spaceflight promotes biofilm formation by Pseudomonas aeruginosa. PLoS ONE, 8(4), e62437. https://doi.org/10.1371/journal.pone.0062437
Kim, J. K., Yun, H., Yeom, C. H., Kim, E. J., Kim, W., Lee, C. S., Kim, B. G., & Jeong, H. J. (2022). Flow cytometry-based rapid detection of Staphylococcus aureus and Pseudomonas aeruginosa using fluorescent antibodies. RSC advances, 12(53), 34660–34669. https://doi.org/10.1039/d2ra05694a
Klintworth, R., Reher, H. J., Viktorov, A. N., & Bohle, D. (1999). Biological induced corrosion of materials II: new test methods and experiences from MIR station. Acta astronautica, 44(7-12), 569-578. https://doi.org/10.1016/S0094-5765(99)00069-7
Kminek, G., Conley, C., Hipkin, V., & Yano, H. (2017). COSPAR’s planetary protection policy. Space Research Today, 200, 12-25.
La Duc, M. T., Kern, R., & Venkateswaran, K. (2004). Microbial monitoring of spacecraft and associated environments. Microbial ecology, 47(2), 150-158.
Larkin, E., Schuerger, A., Barker, R., Lee, J. A., Haveman, N., Enciso, J. F., ... & Espinoza, J. L. (2021). Ground and Flight-Based Plant Microbial Interaction Research and Related Space Crop Production Applications.
Lasseur, C., Brunet, J., De Weever, H., Dixon, M., Dussap, G., Godia, F., ... & Van Der Straeten, D. (2010). MELiSSA: the European project of closed life support system. Gravitational and Space Research, 23(2).
Marra, D., Ferraro, R., & Caserta, S. (2024). Biofilm contamination in confined space stations: Reduction, coexistence or an opportunity? Frontiers in Materials, 11, 1374666. https://doi.org/10.3389/fmats.2024.1374666
Massa, G., Dufour, N., Carver, J., Hummerick, M., Wheeler, R., Morrow, R. & Smith, T. (2017). VEG-01: Veggie Hardware Validation Testing on the International Space Station. Open Agriculture, 2(1), 33-41. https://doi.org/10.1515/opag-2017-0003
Mastroleo, F., Van Houdt, R., Leroy, B., Benotmane, M. A., Janssen, A., Mergeay, M., Vanhavere, F., Hendrickx, L., Wattiez, R., & Leys, N. (2009). Experimental design and environmental parameters affect Rhodospirillum rubrum S1H response to space flight. The ISME journal, 3(12), 1402–1419. https://doi.org/10.1038/ismej.2009.74
McLean, R. J., Cassanto, J. M., Barnes, M. B., & Koo, J. H. (2001). Bacterial biofilm formation under microgravity conditions. FEMS microbiology letters, 195(2), 115–119. https://doi.org/10.1111/j.1574-6968.2001.tb10507.x
Menezes, A. A., Cumbers, J., Hogan, J. A., & Arkin, A. P. (2015). Towards synthetic biological approaches to resource utilization on space missions. Journal of the Royal Society Interface, 12, 20140715. https://doi.org/10.1098/rsif.2014.0715
Mora, M., Wink, L., Kögler, I., Mahnert, A., Rettberg, P., Schwendner, P., Demets, R., Cockell, C., Alekhova, T., Klingl, A., Krause, R., Zolotariof, A., Alexandrova, A., & Moissl-Eichinger, C. (2019). Space Station conditions are selective but do not alter microbial characteristics relevant to human health. Nature communications, 10(1), 3990. https://doi.org/10.1038/s41467-019-11682-z
Morrison, M. D., Thissen, J. B., Karouia, F., Mehta, S., Urbaniak, C., Venkateswaran, K., Smith, D. J., & Jaing, C. (2021). Investigation of Spaceflight Induced Changes to Astronaut Microbiomes. Frontiers in microbiology, 12, 659179. https://doi.org/10.3389/fmicb.2021.659179
Nickerson, C. A., Ott, C. M., Mister, S. J., Morrow, B. J., Burns-Keliher, L., & Pierson, D. L. (2000). Microgravity as a novel environmental signal affecting Salmonella enterica serovar Typhimurium virulence. Infection and immunity, 68(6), 3147–3152. https://doi.org/10.1128/IAI.68.6.3147-3152.2000
Nicholson, W. L., Schuerger, A. C., & Setlow, P. (2009). The solar UV environment and bacterial spore UV resistance: Considerations for planetary protection and Earth-to-Mars transport by natural processes. Mutation Research/Reviews in Mutation Research, 571(1–2), 249–264. https://doi.org/10.1016/j.mrrev.2004.10.001
Niederdorfer, R., Besemer, K., Battin, T. J., & Peter, H. (2017). Ecological strategies and metabolic trade-offs of complex environmental biofilms. NPJ biofilms and microbiomes, 3, 21. https://doi.org/10.1038/s41522-017-0029-y
Novikova, N., De Boever, P., Poddubko, S., Deshevaya, E., Polikarpov, N., Rakova, N., Coninx, I., & Mergeay, M. (2006). Survey of environmental biocontamination on board the International Space Station. Research in microbiology, 157(1), 5–12. https://doi.org/10.1016/j.resmic.2005.07.010
Paradiso, R., De Micco, V., Buonomo, R., Aronne, G., Barbieri, G., & De Pascale, S. (2014). Soilless cultivation of soybean for Bioregenerative Life-Support Systems: a literature review and the experience of the MELiSSA Project - Food characterisation Phase I. Plant biology (Stuttgart, Germany), 16 Suppl 1, 69–78. https://doi.org/10.1111/plb.12056
Percival, S. L., Malic, S., Cruz, H., & Williams, D. W. (2011). Introduction to biofilms. En Biofilms and veterinary medicine (pp. 41–68). Springer.
Philipp, L. A., Bühler, K., Ulber, R., & Gescher, J. (2024). Beneficial applications of biofilms. Nature reviews. Microbiology, 22(5), 276–290. https://doi.org/10.1038/s41579-023-00985-0
Rummel, J. D., Beaty, D. W., Jones, M. A., Bakermans, C., Barlow, N. G., Boston, P. J., … Viso, M. (2014). A new analysis of Mars “special regions”: Findings of the second MEPAG Special Regions Science Analysis Group (SR-SAG2). Astrobiology, 14(11), 887–968. https://doi.org/10.1089/ast.2014.1227
Schuerger, A. C. (1998). Microbial contamination of advanced life support (ALS) systems poses a moderate threat to the long-term stability of space-based bioregenerative systems. Life Support & Biosphere Science, 5(3), 325-337.
Satoh, K., et al. (2011). Fungal contamination in the International Space Station Kibo module. Microbiology and Immunology, 55, 611–618.
Sepe, F., Costanzo, E., Ionata, E., & Marcolongo, L. (2025). Biotechnological Potential of Extremophiles: Environmental Solutions, Challenges, and Advancements. Biology, 14(7), 847. https://doi.org/10.3390/biology14070847
Urbaniak, C., Morrison, M. D., Thissen, J. B., Karouia, F., Smith, D. J., Mehta, S., ... & Venkateswaran, K. (2022). Microbial Tracking-2, a metagenomics analysis of bacteria and fungi onboard the International Space Station. Microbiome, 10(1), 100. https://doi.org/10.1186/s40168-022-01293-0
Venkateswaran, K., Vaishampayan, P., Cisneros, J., Pierson, D. L., Rogers, S. O., & Perry, J. (2014). International Space Station environmental microbiome—Microbial inventories of ISS filter debris. Applied Microbiology and Biotechnology, 98(14), 6453–6466. https://doi.org/10.1007/s00253-014-5650-6
Verseux, C., Baqué, M., Lehto, K., de Vera, J. P. P., Rothschild, L. J., & Billi, D. (2016). Sustainable life support on Mars–the potential roles of cyanobacteria. International Journal of Astrobiology, 15(1), 65-92. https://doi.org/10.1017/S147355041500021X
Wilson, J. W., Ott, C. M., Ramamurthy, R., Porwollik, S., McClelland, M., Pierson, D. L., & Nickerson, C. A. (2002). Low-Shear modeled microgravity alters the Salmonella enterica serovar typhimurium stress response in an RpoS-independent manner. Applied and environmental microbiology, 68(11), 5408–5416. https://doi.org/10.1128/AEM.68.11.5408-5416.2002
Wilson, J. W., Ott, C. M., Höner zu Bentrup, K., Ramamurthy, R., Quick, L., Porwollik, S., Cheng, P., McClelland, M., Tsaprailis, G., Radabaugh, T., Hunt, A., Fernandez, D., Richter, E., Shah, M., Kilcoyne, M., Joshi, L., Nelman-Gonzalez, M., Hing, S., Parra, M., Dumars, P., … Nickerson, C. A. (2007). Space flight alters bacterial gene expression and virulence and reveals a role for global regulator Hfq. Proceedings of the National Academy of Sciences of the United States of America, 104(41), 16299–16304. https://doi.org/10.1073/pnas.0707155104
Yadav, N., Kour, D., & Yadav, A. N. (2018). Microbiomes of freshwater lake ecosystems. J Microbiol Exp, 6(6), 245-248. https://doi.org/10.15406/jmen.2018.06.00223
Zea, L., Larsen, M., Estante, F., Qvortrup, K., Moeller, R., Dias de Oliveira, S., ... & Klaus, D. (2017). Phenotypic changes exhibited by E. coli cultured in space. Frontiers in Microbiology, 8, 1598. https://doi.org/10.3389/fmicb.2017.01598
Zea, L., McLean, R. J., Rook, T. A., Angle, G., Carter, D. L., Delegard, A., ... & Justiniano, Y. A. V. (2020). Potential biofilm control strategies for extended spaceflight missions. Biofilm, 2, 100026. https://doi.org/10.1016/j.bioflm.2020.100026
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